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The enzyme-linked immune sorbent assay (ELISA) is a commonly used analytical biochemistry assay. The assay uses a solid-phase type of enzyme immunoassay (EIA) to detect the presence of a ligand (commonly a protein) in a liquid sample using antibodies directed against the protein to measure. ELISA is already in use as a diagnostic tool in medicine, plant pathology, and biotechnology, as well as quality control, check-in in various industries. This course briefly explains the meaning, basic principles, types, and application of ELISA. One common use of the ELISA tests is to determine if the patient has antibodies related to certain infectious conditions. Antibodies are proteins that our body produces in response to harmful substances called antigens. For instance, an ELISA test can be useful to diagnose HIV (which causes AIDS), Lyme disease, pernicious anemia, rotavirus, syphilis, varicella-zoster virus (which causes chickenpox), Zika virus, etc. Any person with a biological science background would get a primary but clear concept of ELISA after watching this video. Therefore, this course will be particularly helpful for the students of Pharmacy, Biochemistry, and Biotechnology. Also, people who are working in the field of Microbiology, Molecular Biology, Genetics & Bioinformatics, or any other health science-related subject will get benefitted from this course.
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